Four people were sprayed with heating oil and had to take emergency decontamination showers.
The Lincoln Tunnel shutdown snarled traffic Monday evening.
The oil spill in the Kill Van Kull had no impact on Shooters Island Bird Sanctuary, officials said.
The U.S. Coast Guard said 2,900 birds are in the affected area.
A barge spilled fuel oil into the Kill Van Kull, which spread to Shooter's Island, on Friday.
Firefighters were on the scene late Wednesday night helping to clean up the spill.
Fire department officials said the spill has been contained and no one was injured.
A gasoline truck was involved in a crash on the BQE in Astoria that caused a fuel spill Wednesday.
FDNY and HazMat crews responded after 30 gallons of fuel oil spilled on the street in front of 3 Park Row.
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